Analysing Cells

5 Parts:

Electron Microscope

Differential Centrifugation

Cell Differentiation

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In order to make all of the discoveries explained in cell structure, scientists first needed to use a variety of techniques. These are all explained on this page.

An electron microscope is a very expensive and a very large piece of equipment that is very different to a light microscope you will have used in the lab. It is capable of magnifications up to millions of times (compared to hundreds with a light microscope).

There are two types of electron microscopes:

1. 1
   The transmission electron microscope (TEM)

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   The TEM works by passing electrons through a specimen that is no more than 1µm (one micrometer) thick. The electrons in the specimen are diffracted and the electrons are then picked up by a fluorescent screen.

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2. 2
   Scanning electron microscope (SEM)

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   The SEM requires a mould to be made of the specimen using a heavy metal. Electrons are then reflected off of this sample. The advantage of the SEM is that it creates more 3D images, but the resolution and magnification is less.

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You can see some examples of what several organelles look like under an electron microscope if you scroll down

This is a method to separate different organelles of a cell so they can be analyzed, perhaps to look at under an electron microscope.

The first image shows a sample that has been cut up into smaller pieces in an ice-cold buffer solution. This solution maintains the pH so that enzymes will not denature, and the ice prevents any of the enzymes breaking down newly exposed parts of the cell. In the second image you can see the sample being homogenized; the sample is put into a homogenizer (a bit like a blender) which breaks up the cells.

The mixture is then filtered to remove debris and poured into test tubes that are placed in a centrifuge where it is spun at very high speeds. The most dense material forms a pellet at the bottom of this sample (probably the nucleus). A liquid sitting on top of the pellet also forms. This is called the supernatant. Once it has been separated, the supernatant can then be spun to separate the next densest material.

Differentiation means to be able to tell the difference between different things. It is important to be able to do this when analyzing cells, so you know what organelles you are looking at. For information on them, see cell structure.

Nucleus	Rough Endoplasmic Reticulum	Golgi Body
Mitochondrion	Chloroplast	Vacuole

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APA (American Psychological Association)
Analysing Cells. (2017). In ScienceAid. Retrieved Sep 30, 2023, from https://scienceaid.net/biology/cell/analysingcells.html

MLA (Modern Language Association) "Analysing Cells." ScienceAid, scienceaid.net/biology/cell/analysingcells.html Accessed 30 Sep 2023.

Chicago / Turabian ScienceAid.net. "Analysing Cells." Accessed Sep 30, 2023. https://scienceaid.net/biology/cell/analysingcells.html.

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